CGRP Recombinant Antibody [PSH08-45] - Rat IgG1 (Chimeric)
cat.: HA601423
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, WB |
| Clone number: | PSH08-45 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Immunogen: | Recombinant protein within rat CGRP aa 1-128. |
| Positive control: | Mouse colon, mouse spinal cord tissue, rat spinal cord tissue, TT cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:500 1:2,000 1:2,000 |
| Uniprot #: | SwissProt: P06881 Human | P10092 Human | P70160 Mouse | Q99JA0 Mouse | P01256 Rat | P01257 Rat |
| Alternative names: | Calcitonin/calcitonin related polypeptide alpha Alpha type CGRP Beta type CGRP CALC1 CALC2 CALCA CALCB Calcitonin 1 Calcitonin 2 Calcitonin gene related peptide I Calcitonin gene related peptide II Calcitonin related polypeptide alpha Calcitonin related polypeptide beta CGRP CGRP I CGRP II CGRP1 CGRP2 CT Katacalcin KC |
Images
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Fig1:
Immunofluorescence analysis of frozen mouse colon tissue with Rat anti-CGRP antibody (HA601423) at 1/500 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601423, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue with Rat anti-CGRP antibody (HA601423) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601423) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue with Rat anti-CGRP antibody (HA601423) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601423) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Western blot analysis of CGRP on different lysates with Rat anti-CGRP antibody (HA601423) at 1/2,000 dilution. Lane 1: TT cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 10 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601423) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.