HLA Class I Recombinant Mouse Monoclonal Antibody [PSH12-15]
cat.: HA601424
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH12-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG1 |
| Positive control: | Jurkat. |
| Subcellular location: | Cell membrane, Endoplasmic reticulum membrane. |
| Recommended Dilutions:
IF-Cell FC |
1:100 1:1,000 |
| Uniprot #: | SwissProt: P04439 Human | P01889 Human | P10321 Human |
| Alternative names: | Antigen Presenting Molecule Cw*1701 D6S204 FLJ27082 HLA A HLA B HLA C HLA class 1 A HLA class 1 B HLA class 1 C HLA JY3 HLAA HLAB HLAC HLC C Human Leucocyte Antigen C Major Histocompatibility Complex Class I A Major Histocompatibility Complex Class I B Major Histocompatibility Complex Class I C Major histocompatibility complex, class I, A + B + C MHC class I antigen A*1 MHC Class I Antigen MHC class I antigen B*7 MHC class I antigen Cw*1 MHC class I HLA A MHC class I HLA B MHC class I HLA C MHC HLA ABC PSORS1 |
Images
|
Fig1:
Immunocytochemistry analysis of Jurkat cells labeling HLA Class I with Mouse anti-HLA Class I antibody (HA601424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HLA Class I antibody (HA601424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
|
Fig2:
Flow cytometric analysis of K-562 (left, negative) and Jurkat (right, positive) cells labeling HLA Class I. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601424, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.