Caspase-4 Mouse Monoclonal Antibody [A9G2]
cat.: HA601431
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Cynomolgus monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A9G2 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human Caspase 4 aa 81-270. |
| Positive control: | THP-1 cell lysate, U-937 cell lysate. |
| Subcellular location: | Cytoplasm, cytosol, Endoplasmic reticulum membrane, Mitochondrion, Inflammasome, Secreted . |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P49662 Human |
| Alternative names: | Apoptotic cysteine protease Mih1/TX CASP-4 CASP4 CASP4_HUMAN Caspase 4 apoptosis related cysteine peptidase Caspase-4 subunit 2 Caspase4 ICE(rel)-II ICE(rel)II ICEREL II ICH2 Mih1/TX Protease ICH-2 Protease TX TX |
Images
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Fig1:
Western blot analysis of Caspase-4 on different lysates with Mouse anti-Caspase-4 antibody (HA601431) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: U-937 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601431) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.