Slc12a5 / KCC2 Recombinant Mouse Monoclonal Antibody [PSH13-20]
cat.: HA601433
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, WB |
| Clonality: | Monoclonal |
| Clone number: | PSH13-20 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 126 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Rat Slc12a5 / KCC2 aa 932-1043. |
| Positive control: | Mouse hippocampus tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cell membrane, Cell projection, dendrite. |
| Recommended Dilutions:
IHC-Fr WB |
1:500 1:5,000 |
| Uniprot #: | SwissProt: Q91V14 Mouse | Q63633 Rat |
| Alternative names: | Electroneutral potassium chloride cotransporter 2 Electroneutral potassium-chloride cotransporter 2 Erythroid K Cl cotransporter 2 Furosemide sensitive K Cl cotransporter hKCC2 K-Cl cotransporter 2 KCC 2 KCC2 KIAA1176 Neuronal K Cl cotransporter Neuronal K-Cl cotransporter Potassium Chloride Cotransporter Potassium chloride transporter 5 rKCC2 S12A5 S12A5_HUMAN SLC12A5 Solute carrier family 12 (potassium chloride transporter) member 5 Solute carrier family 12 member 5 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Rat Site: cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig3:
Application: IHC-Fr Species: Rat Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig4:
Western blot analysis of Slc12a5 / KCC2 on different lysates with Mouse anti-Slc12a5 / KCC2 antibody (HA601433) at 1/5,000 dilution. Lane 1: Mouse hippocampus tissue lysate (no heat) Lane 2: Mouse brain tissue lysate (no heat) Lane 3: Rat brain tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 40 µg/Lane. Predicted band size: 126 kDa Observed band size: 150 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601433) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.