Kv4.2 / KCND2 Recombinant Mouse Monoclonal Antibody [PSH13-21]
cat.: HA601434
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, WB |
| Clonality: | Monoclonal |
| Clone number: | PSH13-21 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Rat Kv4.2 / KCND2 aa 207-226. |
| Positive control: | Mouse cerebellum tissue, rat cerebellum tissue, Mouse cerebellum tissue lysate, Mouse hippocampus tissue lysate, Mouse brain tissue lysate, Rat cerebellum tissue lysate. |
| Subcellular location: | Cell membrane, Cell projection, dendrite, Synapse, Perikaryon, Postsynaptic cell membrane, Cell projection, Cell junction. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:500 1:1,000 1:5,000 |
| Uniprot #: | SwissProt: Q9Z0V2 Mouse | Q63881 Rat |
| Alternative names: | KCD2 KCND 2 KCND2 KCND2_HUMAN KIAA1044 MGC119702 MGC119703 Potassium voltage gated channel Shal related subfamily member 2 Potassium voltage-gated channel subfamily D member 2 RK 5 RK5 Voltage gated potassium channel Kv4.2 Voltage gated potassium channel subunit Kv4.2 Voltage sensitive potassium channel Voltage-gated potassium channel subunit Kv4.2 |
Images
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Fig1:
Application: IHC-Fr Species: Rat Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-Kv4.2 / KCND2 antibody (HA601434) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601434) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-Kv4.2 / KCND2 antibody (HA601434) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601434) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Western blot analysis of Kv4.2 / KCND2 on different lysates with Mouse anti-Kv4.2 / KCND2 antibody (HA601434) at 1/5,000 dilution. Lane 1: Mouse cerebellum tissue lysate (no heat) Lane 2: Mouse hippocampus tissue lysate (no heat) Lane 3: Mouse brain tissue lysate (no heat) Lane 4: Mouse liver tissue lysate (negative) Lane 5: Rat cerebellum tissue lysate Lane 6: Rat liver tissue lysate (negative) Notice: no heat means the lysate is not boiled. Lysates/proteins at 40 µg/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601434) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.