| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH13-22 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 95 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Rat TRPV1 aa 1-200. |
| Positive control: | Rat dorsal root ganglia tissue. |
| Subcellular location: | Postsynaptic cell membrane, Cell projection, dendritic spine membrane, Cell membrane. |
| Recommended Dilutions:
IHC-P |
1:200 |
| Uniprot #: | SwissProt: O35433 Rat |
| Alternative names: | Capsaicin receptor DKFZp434K0220 osm 9 like TRP channel 1 Osm-9-like TRP channel 1 OTRPC1 Transient receptor potential cation channel subfamily V member 1 TRPV 1 Trpv1 TRPV1_HUMAN Vanilloid receptor 1 Vanilloid receptor subtype 1 VR 1 VR1 |
|
Fig1:
Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglia tissue with Mouse anti-TRPV1 antibody (HA601435) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601435) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat skin tissue (negative) with Mouse anti-TRPV1 antibody (HA601435) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601435) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |