p75 NGF Receptor Recombinant Rat Monoclonal Antibody [PSH13-26]
cat.: HA601436
| Product Type: | Recombinant Rat Monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, WB, IF-Cell |
| Clone number: | PSH13-26 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG1 |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cell membrane, Cytoplasm, Perikaryon, Cell projection, growth cone, dendritic spine. |
| Recommended Dilutions:
IHC-Fr WB IF-Cell |
1:200 1:2,000 1:100 |
| Uniprot #: | SwissProt: Q9Z0W1 Mouse | P07174 Rat |
| Alternative names: | CD271 CD271 antigen Gp80 LNGFR Gp80-LNGFR Low affinity nerve growth factor receptor Low affinity neurotrophin receptor p75NTR Low-affinity nerve growth factor receptor Nerve growth factor receptor Nerve growth factor receptor TNFR superfamily member 16 NGF receptor Ngfr p75 ICD p75 Neurotrophin receptor p75 NTR p75(NTR) p75NTR TNFR Superfamily Member 16 TNFRSF16 TNR16_HUMAN Tumor necrosis factor receptor superfamily member 16 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: brain (medial septum) Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Mouse Site: colon Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
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Fig3:
Western blot analysis of p75 NGF Receptor on different lysates with Rat anti-p75 NGF Receptor antibody (HA601436) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 70 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601436) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunocytochemistry analysis of Neuro-2a cells labeling p75 NGF Receptor with Rat anti-p75 NGF Receptor antibody (HA601436) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rat anti-p75 NGF Receptor antibody (HA601436) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.