Human uPAR Mouse Monoclonal Antibody [PSH13-77]
cat.: HA601438
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH13-77 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human uPAR aa 23-303 (HA211102). |
| Positive control: | Recombinant Human uPAR protein (HA211102). |
| Subcellular location: | Cell membrane |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-76] to Human uPAR antibody (Capture) (HA601437) and recombinant Human uPAR protein (HA211102) as the standard. The reference range value is 125-4,000 pg/mL. |
| Uniprot #: | SwissProt: Q03405 Human |
| Alternative names: | CD 87 CD87 CD87 antigen MO 3 MO3 Monocyte activation antigen Mo3 Plasminogen activator receptor urokinase Plasminogen activator urokinase receptor PLAUR U PAR u plasminogen activator receptor U-PAR u-plasminogen activator receptor form 2 UPA receptor uPAR UPAR_HUMAN Urinary plasminogen activator receptor URKR Urokinase plasminogen activator receptor Urokinase plasminogen activator surface receptor urokinase-type plasminogen activator (uPA) receptor |
Images
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Fig1:
Sandwich ELISA analysis of Human uPAR matched pair antibodies Capture: HA601437, Human uPAR Rabbit mAb [PSH13-76] Detector: HA601438, Human uPAR Rabbit mAb [PSH13-77] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA601437) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human uPAR protein (HA211102) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA601438, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.