HA601440

c-Fos Recombinant Antibody [PSH07-51] - Mouse IgG1 (Chimeric)

cat.: HA601440

Product Type:	Recombinant Chimeric Antibody, primary antibodies
Species reactivity:	Human, Mouse, Rat, Cynomolgus monkey, Pig
Applications:	IHC-Fr, IHC-P, IF-Cell, WB
Clone number:	PSH07-51

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 41 kDa
Immunogen:	Recombinant protein within human Protein c-Fos aa 1-380.
Positive control:	Mouse brain tissue, rat brain tissue.
Subcellular location:	Nucleus, Endoplasmic reticulum, Cytoplasm, cytosol.
Recommended Dilutions: IHC-Fr IHC-P IF-Cell WB	1:1,000-1:5,000 1:5,000 1:1,000 1:5,000-1:10,000
Uniprot #:	SwissProt: P01100 Human \| P01101 Mouse \| P12841 Rat
Alternative names:	Activator protein 1 AP 1 C FOS Cellular oncogene c fos Cellular oncogene fos FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) FBJ murine osteosarcoma viral oncogene homolog FBJ murine osteosarcoma viral v fos oncogene homolog FBJ Osteosarcoma Virus FOS FOS protein FOS_HUMAN G0 G1 switch regulatory protein 7 G0/G1 switch regulatory protein 7 G0S7 Oncogene FOS p55 proto oncogene c Fos Proto oncogene protein c fos Proto-oncogene c-Fos v fos FBJ murine osteosarcoma viral oncogene homolog

Images

	Fig1: Application: IHC-Fr Species: Mouse Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/1,000 (c-Fos, HA601440, Mouse, red); 1/1,000 (Iba1, ET1705-78, Rabbit, green) Antigen retrieval: Not required Important Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.
	Fig2: Application: IHC-Fr Species: Rat Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/1,000 (c-Fos, HA601440, Mouse, red); 1/1,000 (Iba1, ET1705-78, Rabbit, green) Antigen retrieval: Not required Important Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.
	Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-c-Fos antibody (HA601440) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601440) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-c-Fos antibody (HA601440) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601440) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig5: Immunocytochemistry analysis of HeLa cells untreated / HeLa cells starved 16 hours then treated with 200nM TPA for 4 hours labeling c-Fos with Mouse anti-c-Fos antibody (HA601440) at 1/1,000 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-c-Fos antibody (HA601440) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Fig6: Immunocytochemistry analysis of C6 cells untreated / C6 cells starved 16 hours then treated with 200nM TPA for 4 hours labeling c-Fos with Mouse anti-c-Fos antibody (HA601440) at 1/1,000 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-c-Fos antibody (HA601440) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Fig7: Western blot analysis of c-Fos on different lysates with Mouse anti-c-Fos antibody (HA601440) at 1/5,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: RAW264.7 serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 41 kDa
Observed band size: 41-55 kDa

Exposure time: 59 seconds; ECL: K1801;

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601440) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.