MTAP Recombinant Mouse Monoclonal Antibody [PSH13-98]
cat.: HA601445
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH13-98 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 0.375ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG1 |
| Positive control: | HCT 116 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, NIH/3T3, C6. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:50 |
| Uniprot #: | SwissProt: Q13126 Human | Q9CQ65 Mouse Entrez Gene: 298227 Rat |
| Alternative names: | 5' methylthioadenosine phosphorylase 5''-methylthioadenosine phosphorylase BDMF c86fus DMSFH DMSMFH Epididymis luminal protein 249 HEL 249 LGMBF MeSAdo phosphorylase Methylthioadenosine phosphorylase MSAP MTA phosphorylase MTAP MTAP_HUMAN MTAPase S methyl 5 thioadenosine phosphorylase S methyl 5' thioadenosine phosphorylase S-methyl-5''-thioadenosine phosphorylase |
Images
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Fig1:
Western blot analysis of MTAP on different lysates with Mouse anti-MTAP antibody (HA601445) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HeLa cell lysate Lane 3: A549 cell lysate (negative) Lane 4: NIH/3T3 cell lysate Lane 5: C2C12 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601445) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of NIH/3T3 cells labeling MTAP with Mouse anti-MTAP antibody (HA601445) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MTAP antibody (HA601445) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunocytochemistry analysis of C6 cells labeling MTAP with Mouse anti-MTAP antibody (HA601445) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MTAP antibody (HA601445) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.