ROR1 Recombinant Mouse Monoclonal Antibody [PSH13-99]
cat.: HA601447
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH13-99 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 104 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human ROR1 aa 428-937. |
| Positive control: | PANC-1 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, NCCIT cell lysate, K-562 cell lysate, K-562 (left, negative) and NCCIT (right, positive). |
| Subcellular location: | Membrane, Cell projection, axon. |
| Recommended Dilutions:
WB FC |
1:5,000 1:1,000 |
| Uniprot #: | SwissProt: Q01973 Human |
| Alternative names: | dJ537F10.1 Inactive tyrosine protein kinase transmembrane receptor ROR1 MGC99659 Neurotrophic tyrosine kinase Neurotrophic tyrosine kinase receptor related 1 Neurotrophic tyrosine kinase, receptor related 1 NTRKR1 OTTHUMP00000010573 OTTHUMP00000010574 OTTMUSP00000008344 Receptor tyrosine kinase like orphan receptor 1 receptor-related 1 RGD1559469 ROR 1 ROR1 ROR1_HUMAN RP11 24J23.1 Tyrosine kinase like orphan receptor 1 Tyrosine protein kinase transmembrane receptor ROR1 Tyrosine-protein kinase transmembrane receptor ROR1 |
Images
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Fig1:
Western blot analysis of ROR1 on different lysates with Rabbit anti-ROR1 antibody (HA601447) at 1/5,000 dilution. Lane 1: PANC-1 cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (negative) (20 µg/Lane) Lane 3: MDA-MB-231 cell lysate (20 µg/Lane) Lane 4: NCCIT cell lysate (20 µg/Lane) Lane 5: K-562 cell lysate (negative) (20 µg/Lane) Predicted band size: 104 kDa Observed band size: 130 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601447) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of K-562 (left, negative) and NCCIT (right, positive) cells labeling ROR1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA601447, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.