VGLUT1 Recombinant Antibody [PSH10-53] - Guinea pig IgG2 (Chimeric)
cat.: HA601449
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, WB, IF-Tissue |
| Clone number: | PSH10-53 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Immunogen: | Recombinant protein within Mouse VGLUT1 aa 1-560. |
| Positive control: | Mouse brain tissue lysate. |
| Subcellular location: | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cell membrane, Synapse, synaptosome. |
| Recommended Dilutions:
IHC-Fr WB IF-Tissue |
1:500 1:5,000 1:500 |
| Uniprot #: | SwissProt: Q3TXX4 Mouse | Q62634 Rat |
| Alternative names: | BNPI Brain specific Na (+) dependent inorganic phosphate cotransporter Brain specific Na dependent inorganic phosphate cotransporter Brain-specific Na(+)-dependent inorganic phosphate cotransporter Slc17a7 Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 Solute carrier family 17 member 7 Vesicular glutamate transporter 1 VGLU1_HUMAN VGLUT 1 VGluT1 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 (VGLUT1, HA601449, Guinea pig, red); 1/500 (VGLUT2, HA601451, Mouse, green) Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Rat Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 (VGLUT1, HA601449, Guinea pig, red); 1/500 (VGLUT2, HA601451, Mouse, green) Antigen retrieval: Not required |
|
Fig3:
Western blot analysis of VGLUT1 on different lysates with Guinea pig anti-VGLUT1 antibody (HA601449) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 2: Mouse lung tissue lysate (no heat) (negative) (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601449) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/5,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Application: Immunofluorescence (IF-tissue) Species: Mouse Tissue: Hippocampus Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: VGLUT1, 1/500 (HA601449, Guinea pig, Red), GAD67, 1:500 (HA601370, Mouse, Green), overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), Goat anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature. |
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Fig5:
Application: Immunofluorescence (IF-tissue) Species: Rat Tissue: Hippocampus Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: VGLUT1, 1/500 (HA601449, Guinea pig, Red), GAD67, 1:500 (HA601370, Mouse, Green), overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), Goat anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.