GAD65 Recombinant Antibody [PSH06-80] - Mouse IgG1 (Chimeric)
cat.: HA601455
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, WB |
| Clone number: | PSH06-80 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 65 kDa |
| Positive control: | Mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol, Cytoplasmic vesicle, Presynaptic cell membrane, Golgi apparatus membrane. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:200 1:1,000 1:5,000 |
| Uniprot #: | SwissProt: P48320 Mouse | Q05683 Rat |
| Alternative names: | 65 kDa glutamic acid decarboxylase DCE 2 DCE2 DCE2_HUMAN GAD 2 GAD 65 GAD-2 GAD-65 Gad2 Glutamate decarboxylase 2 (pancreas) Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa) Glutamate decarboxylase 2 Glutamate Decarboxylase 65 Glutamate decarboxylase 65 kDa isoform Glutamic Acid Decarboxylase 2 Glutamic Acid Decarboxylase 65 MGC161605 MGC161607 |
Images
|
Fig1:
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-GAD65 antibody (HA601455) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-GAD65 antibody (HA601455) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-GAD65 antibody (HA601455) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-GAD65 antibody (HA601455) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Western blot analysis of GAD65 on different lysates with Mouse anti-GAD65 antibody (HA601455) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601455) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.