IP3 receptor Recombinant Mouse Monoclonal Antibody [PSH14-18]
cat.: HA601456
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH14-18 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 313 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Rat IP3 receptor aa 2591-2750 (Cytoplasmic). |
| Positive control: | Mouse brain tissue lysate, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Endoplasmic reticulum membrane, Cytoplasmic vesicle, secretory vesicle membrane, Cytoplasm, perinuclear region. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:1,000 |
| Uniprot #: | SwissProt: P11881 Mouse | P29994 Rat |
| Alternative names: | 4 5-trisphosphate receptor 5-trisphosphate receptor type 1 DKFZp313E1334 DKFZp313N1434 inositol 1 4 5 triphosphate receptor type 1 Inositol 1 4 5 trisphosphate Receptor Type 1 Inositol 1 InsP3R1 IP3 IP3 receptor IP3 receptor isoform 1 IP3R 1 IP3R IP3R1 ITPR 1 Itpr1 ITPR1_HUMAN SCA15 SCA16 SCA29 Type 1 inositol 1 4 5 trisphosphate receptor Type 1 inositol 1 Type 1 InsP3 receptor |
Images
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Fig1:
Western blot analysis of IP3 receptor on different lysates with Mouse anti-IP3 receptor antibody (HA601456) at 1/5,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate (negative) (40 µg/Lane) Lane 2: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 313 kDa Observed band size: 313 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601456) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-IP3 receptor antibody (HA601456) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601456) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-IP3 receptor antibody (HA601456) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601456) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.