Nestin Recombinant Antibody [PSH07-89] - Guinea pig IgG2 (Chimeric)
cat.: HA601468
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-Fr, WB |
| Clone number: | PSH07-89 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 207 kDa |
| Immunogen: | Recombinant protein within mouse Nestin aa 700-1,000. |
| Positive control: | RD cell lysate, C2C12 cell lysate, C6 cell lysate. |
| Subcellular location: | Cytoplasm, Intermediate filament. |
| Recommended Dilutions:
IHC-Fr WB |
1:200 1:10,000 |
| Uniprot #: | SwissProt: P48681 Human | Q6P5H2 Mouse | P21263 Rat |
| Alternative names: | ESTM 46 FLJ 21841 FLJ21841 Intermediate filament protein Nbla00170 nes NEST_HUMAN Nestin |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: embryonic cartilage Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
|
Fig2:
Application: IHC-Fr Species: Mouse Site: kidney Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
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Fig3:
Application: IHC-Fr Species: Rat Site: kidney Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
|
Fig4:
Western blot analysis of Nestin on different lysates with Guinea pig anti-Nestin antibody (HA601468) at 1/10,000 dilution. Lane 1: RD cell lysate Lane 2: 293T cell lysate (negative) Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 207 kDa Observed band size: 100-300 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601468) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.