NeuN Recombinant Antibody [SR45-07] - Guinea pig IgG2 (Chimeric)
cat.: HA601481
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, WB |
| Clone number: | SR45-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Immunogen: | Synthetic peptide within human NeuN aa 20-60. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
IHC-Fr WB |
1:200 1:10,000 |
| Uniprot #: | SwissProt: Q8BIF2 Mouse Unigene: 143966 Rat |
| Alternative names: | FLJ56884 FLJ58356 Fox-1 homolog C fox1 homolog C Fox3 FOX3NeuN hexaribonucleotide binding protein 3 HRNBP3 NEUN neuronal nuclei Rbfox3 RFOX3_HUMAN RNA binding protein fox-1 homolog 3 RNA binding protein, fox 1 homolog (C. elegans) 3 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
|
Fig2:
Western blot analysis of NeuN on different lysates with Guinea pig anti-NeuN antibody (HA601481) at 1/10,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: C2C12 cell lysate (negative) Lane 3: Rat brain tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 34 kDa Observed band size: 45/50 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601481) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.