| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, WB |
| Clone number: | PSH10-32 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 8 kDa |
| Immunogen: | Recombinant protein within human aa 1-73. |
| Positive control: | Mouse lung tissue lysate, Mouse cerebellum tissue lysate, Rat lung tissue lysate. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
IHC-Fr WB |
1:200 1:2,000 |
| Uniprot #: | SwissProt: Q8R1H0 Mouse | Q78ZR5 Rat |
| Alternative names: | CAMEO heart odd homeobox 1 protein HOD Homeodomain-only protein HOP HOP homeobox HOP_HUMAN HOPX LAGY Lung cancer-associated Y protein NECC1 Not expressed in choriocarcinoma clone 1 Not expressed in choriocarcinoma protein 1 OB1 odd homeobox 1 protein Odd homeobox protein 1 OTTHUMP00000158970 SMAP31 TOTO |
|
Fig1:
Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
|
Fig2:
Western blot analysis of HOPX / HOD on different lysates with Rat anti-HOPX / HOD antibody (HA601484) at 1/2,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse liver tissue lysate (low expression) Lane 4: Rat lung tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 8 kDa Observed band size: 14 kDa Exposure time: 2 minutes 41 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601484) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature. |