HOPX / HOD Recombinant Antibody [PSH10-32] - Rat IgG1 (Chimeric)
cat.: HA601484
Product Type: Recombinant Chimeric Antibody, primary antibodies
Species reactivity: Mouse, Rat
Applications: IHC-Fr, WB
Clone number: PSH10-32
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 8 kDa
Immunogen: Recombinant protein within human aa 1-73.
Positive control: Mouse lung tissue lysate, Mouse cerebellum tissue lysate, Rat lung tissue lysate.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  IHC-Fr
  WB

1:200
1:2,000
Uniprot #: SwissProt: Q8R1H0 Mouse | Q78ZR5 Rat
Alternative names: CAMEO heart odd homeobox 1 protein HOD Homeodomain-only protein HOP HOP homeobox HOP_HUMAN HOPX LAGY Lung cancer-associated Y protein NECC1 Not expressed in choriocarcinoma clone 1 Not expressed in choriocarcinoma protein 1 OB1 odd homeobox 1 protein Odd homeobox protein 1 OTTHUMP00000158970 SMAP31 TOTO
Images
HA601484_1.jpg Fig1: Application: IHC-Fr

Species: Mouse

Site: cerebellum

Sample: Frozen section

Antibody concentration: 1/200

Antigen retrieval: Not required
HA601484_2.jpg Fig2: Western blot analysis of HOPX / HOD on different lysates with Rat anti-HOPX / HOD antibody (HA601484) at 1/2,000 dilution.

Lane 1: Mouse lung tissue lysate
Lane 2: Mouse cerebellum tissue lysate
Lane 3: Mouse liver tissue lysate (low expression)
Lane 4: Rat lung tissue lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 8 kDa
Observed band size: 14 kDa

Exposure time: 2 minutes 41 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601484) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.