CD25 / IL-2 Receptor alpha Recombinant Mouse Monoclonal Antibody [PSH14-46]
cat.: HA601486
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | FC, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH14-46 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 1% BSA, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human CD25 aa 22-240 (Extracellular). |
| Positive control: | Human peripheral blood, mouse spleen tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
FC IHC-P IF-Tissue |
1:1,000 (For concentrate), 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood (For predilute) 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P01589 Human | P01590 Mouse |
| Alternative names: | Interleukin 2 receptor alpha chain CD25 CD25 antigen IDDM10 IL 2 receptor alpha subunit IL-2 receptor subunit alpha IL-2-RA IL-2R subunit alpha IL2 RA IL2 Receptor alpha IL2-RA IL2R IL2R, alpha chain IL2RA IL2RA_HUMAN IMD41 Interleukin 2 receptor alpha Interleukin 2 receptor Interleukin-2 receptor subunit alpha p55 t-cell growth factor receptor TAC TAC antigen TCGFR |
Images
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Fig1:
Flow cytometric analysis of human peripheral blood cells labeling CD25 / IL-2 Receptor alpha (HA601486) and CD4 (HA600121F, APC). Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601486, 1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Compared with Mouse IgG Isotype Control (iFluor™ 488, 1/1,000). |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-CD25 / IL-2 Receptor alpha antibody (HA601486) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601486) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Application: IF-Tissue Species: Mouse Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/1,000 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.