EAAT2 Recombinant Antibody [PS01-62] - Guinea pig IgG2 (Chimeric)
cat.: HA601504
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, WB |
| Clone number: | PS01-62 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Immunogen: | Synthetic peptide within Human EAAT2 |
| Positive control: | Mouse brain tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IHC-Fr WB |
1:200 1:5,000 |
| Uniprot #: | SwissProt: P43006 Mouse | P31596 Rat |
| Alternative names: | EAA2_HUMAN EAAT2 Excitatory amino acid transporter 2 Excitotoxic amino acid transporter 2 Glial high affinity glutamate transporter GLT 1 GLT1 Glutamate aspartate transporter II Glutamate transporter 1 Glutamate/aspartate transporter II Slc1a2 Sodium dependent glutamate aspartate transporter 2 Sodium-dependent glutamate/aspartate transporter 2 solute carrier family 1 (glial high affinity glutamate transporter), member 2 Solute carrier family 1 glial high affinity glutamate transporter member 2 Solute carrier family 1 member 2 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
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Fig2:
Western blot analysis of EAAT2 on different lysates with Guinea pig anti-EAAT2 antibody (HA601504) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) Lane 2: Mouse lung tissue lysate (negative) (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601504) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.