SIRT2 Recombinant Antibody [SN70-04] - Rat IgG1 (Chimeric)
cat.: HA601506
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, WB |
| Clone number: | SN70-04 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Immunogen: | Synthetic peptide within Human SIRT2 aa 335-389 / 389. |
| Positive control: | Mouse brain (striatum) tissue, rat brain (striatum) tissue. |
| Subcellular location: | Nucleus, Cytoplasm, Midbody, Chromosome, Cell projection, Myelin membrane. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:500 1:2,000 1:10,000 |
| Uniprot #: | SwissProt: Q8VDQ8 Mouse | Q5RJQ4 Rat |
| Alternative names: | FLJ35621 FLJ37491 NAD dependent deacetylase sirtuin 2 NAD-dependent deacetylase sirtuin-2 NAD-dependent protein deacetylase sirtuin-2 Regulatory protein SIR2 homolog 2 Silencing information regulator 2 like Silent information regulator 2 SIR2 SIR2 like protein 2 Sir2 related protein type 2 SIR2, S. cerevisiae, homolog-loke 2 SIR2-like protein 2 SIR2L SIR2L2 SIRT2 SIRT2_HUMAN Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) Sirtuin 2 Sirtuin type 2 |
Images
|
Fig1:
Application: IHC-Fr Species: Mouse Site: brain (striatum) Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain (striatum) tissue with Rat anti-SIRT2 antibody (HA601506) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601506) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain (striatum) tissue with Rat anti-SIRT2 antibody (HA601506) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601506) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.