MAG Recombinant Antibody [PSH02-41] - Guinea pig IgG2 (Chimeric)
cat.: HA601510
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, WB |
| Clone number: | PSH02-41 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Immunogen: | Recombinant protein within human MAG aa 1-536 / 626. |
| Positive control: | Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate. |
| Subcellular location: | Cell membrane, Membrane raft. |
| Recommended Dilutions:
IHC-Fr WB |
1:200 1:5,000 |
| Uniprot #: | SwissProt: P20917 Mouse | P07722 Rat |
| Alternative names: | GMA MAG MAG_HUMAN Myelin associated glycoprotein Myelin-associated glycoprotein S MAG S-MAG Sialic acid binding Ig like lectin 4A Sialic acid binding immunoglobulin like lectin 4A Siglec 4a Siglec-4a SIGLEC4A SPG75 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
|
Fig2:
Western blot analysis of MAG on different lysates with Guinea pig anti-MAG antibody (HA601510) at 1/10,000 dilution. Lane 1: Mouse cerebellum tissue lysate Lane 2: Mouse liver tissue lysate (negative) Lane 3: Rat cerebellum tissue lysate Lane 4: Rat liver tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601510) at 1/10,000 dilution was used in primary antibody dilution (K1803) at room temperature for 2 hours. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.