HA601514

PGP9.5 Recombinant Antibody [JM10-59] - Mouse IgG1 (Chimeric)

cat.: HA601514

Product Type:	Recombinant Chimeric Antibody, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	IHC-Fr, IF-Tissue, IHC-P, WB, IF-Cell
Clone number:	JM10-59

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 25 kDa
Immunogen:	Synthetic peptide within Human PGP95 aa 191-223 / 223.
Positive control:	Human pancreas tissue, rat pancreas tissue, SH-SY5Y cell lysate, A-172 cell lysate, Neuro-2a cell lysate, C6 cell lysate, SH-SY5Y.
Subcellular location:	Cytoplasm, Endoplasmic reticulum membrane.
Recommended Dilutions: IHC-Fr IF-Tissue IHC-P WB IF-Cell	1:200 1:500 1:5,000 1:10,000 1:200
Uniprot #:	SwissProt: P09936 Human \| Q9R0P9 Mouse \| Q00981 Rat
Alternative names:	Epididymis luminal protein 117 Epididymis secretory protein Li 53 HEL 117 HEL S 53 NDGOA Neuron cytoplasmic protein 9.5 OTTHUMP00000218137 OTTHUMP00000218139 OTTHUMP00000218140 OTTHUMP00000218141 Park 5 PARK5 PGP 9.5 PGP9.5 PGP95 Protein gene product 9.5 Ubiquitin C terminal esterase L1 Ubiquitin C terminal hydrolase Ubiquitin C terminal hydrolase L1 Ubiquitin carboxyl terminal esterase L1 Ubiquitin carboxyl terminal hydrolase isozyme L1 Ubiquitin carboxyl-terminal hydrolase isozyme L1 Ubiquitin thioesterase L1 Ubiquitin thiolesterase Ubiquitin thiolesterase L1 UCH-L1 UCHL1 UCHL1_HUMAN

Images

	Fig1: Application: IHC-Fr Species: Rat Site: pancreas Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required
	Fig2: Application: IF-Tissue Species: Human Site: pancreas Sample: Paraffin-embedded section Antibody concentration: 1/500
	Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-PGP9.5 antibody (HA601514) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601514) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Mouse anti-PGP9.5 antibody (HA601514) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601514) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig5: Western blot analysis of PGP9.5 on different lysates with Mouse anti-PGP9.5 antibody (HA601514) at 1/10,000 dilution.

Lane 1: SH-SY5Y cell lysate
Lane 2: A-172 cell lysate
Lane 3: LNCaP cell lysate (negative)
Lane 4: Neuro-2a cell lysate
Lane 5: NIH/3T3 cell lysate (negative)
Lane 6: C6 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 25 kDa
Observed band size: 25 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601514) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig6: Immunocytochemistry analysis of SH-SY5Y cells labeling PGP9.5 with Mouse anti-PGP9.5 antibody (HA601514) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PGP9.5 antibody (HA601514) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.