pan Cytokeratin Recombinant Antibody [PDH09-10] - Rat IgG1 (Chimeric)
cat.: HA601519
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, mIHC |
| Clone number: | PDH09-10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Immunogen: | Full length native protein corresponding to Human pan Cytokeratin. |
| Positive control: | Human stomach carcinoma tissue, human liver tissue. |
| Subcellular location: | Cytoplasmic |
| Recommended Dilutions:
IHC-P mIHC |
1:2,000 1:2,000 |
| Uniprot #: | SwissProt: Q01546 Human | Q7Z794 Human | P48668 Human | P13645 Human |
| Alternative names: | pan Cytokeratin pan-Cytokeratin pan ck pan-ck panck pan Keratin |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rat anti-pan Cytokeratin antibody (HA601519) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601519) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rat anti-pan Cytokeratin antibody (HA601519) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601519) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.