F4/80 Recombinant Antibody [PSH01-87] - Mouse IgG1 (Chimeric)
cat.: HA601537
| Product Type: | Recombinant Chimeric Antibody IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P |
| Clone number: | PSH01-87 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 102 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse F4/80 aa 1-650 / 931. |
| Positive control: | RAW264.7 cell lysate, Mouse spleen tissue lysate, mouse liver tissue, mouse lung tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:2,000-1:5,000 |
| Uniprot #: | SwissProt: Q61549 Mouse |
| Alternative names: | ADGRE1 Adhesion G protein coupled receptor E1 Adhesion G protein-coupled receptor E1 AGRE1_HUMAN Cell surface glycoprotein EMR1 Cell surface glycoprotein F4/80 DD7A5 7 Egf like module containing mucin like hormone receptor like 1 Egf like module containing mucin like hormone receptor like sequence 1 EGF like module receptor 1 EGF TM7 EGF-like module receptor 1 EGF-like module-containing mucin-like hormone receptor-like 1 EGFTM7 EMR 1 EMR1 EMR1 hormone receptor Gpf480 Ly71 Lymphocyte antigen 71 TM7LN3 |
Images
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Fig1:
Western blot analysis of F4/80 on different lysates with Mouse anti-F4/80 antibody (HA601537) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate (no heat) (10 µg/Lane) Lane 2: L-929 cell lysate (negative) (10 µg/Lane) Lane 3: Mouse spleen tissue lysate (no heat) (10 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 102 kDa Observed band size: 150 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601537) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-F4/80 antibody (HA601537) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601537) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Mouse anti-F4/80 antibody (HA601537) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601537) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.