CD163 Recombinant Antibody [JA51-30] - Rat IgG1 (Chimeric)
cat.: HA601549
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, mIHC |
| Clone number: | JA51-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 125 kDa |
| Immunogen: | Recombinant protein within Human CD163 aa 1012-1149 / 1156. |
| Positive control: | Human liver tissue, human placenta tissue, human spleen tissue, human tonsil tissue. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
IHC-P mIHC |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q86VB7 Human |
| Alternative names: | C163A_HUMAN CD 163 CD163 CD163 antigen CD163 molecule Hemoglobin scavenger receptor M130 M130 antigen precursor Macrophage associated antigen MM130 OTTHUMP00000238617 OTTHUMP00000238618 OTTHUMP00000238619 OTTHUMP00000238620 SCARI1 Scavenger receptor cysteine rich type 1 protein M130 sCD163 Soluble CD163 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rat anti-CD163 antibody (HA601549) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601549) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rat anti-CD163 antibody (HA601549) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601549) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rat anti-CD163 antibody (HA601549) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601549) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rat anti-CD163 antibody (HA601549) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601549) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.