| Product Type: | Recombinant Chimeric Antibody IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P |
| Clone number: | SP07-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Olig2 aa 238-287 / 323. |
| Positive control: | Mouse brain tissue, rat brain tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
IHC-Fr IHC-P |
1:200 1:500 |
| Uniprot #: | SwissProt: Q9EQW6 Mouse Unigene: 22121 Rat |
| Alternative names: | Basic domain helix loop helix protein class B 1 Basic helix loop helix protein class B 1 BHLHB bHLHB1 bHLHe19 Class B basic helix loop helix protein 1 Class B basic helix-loop-helix protein 1 class E basic helix loop helix protein 19 Class E basic helix-loop-helix protein 19 Human protein kinase C binding protein RACK17 Olig2 OLIG2_HUMAN Oligo2 Oligodendrocyte lineage transcription factor 2 Oligodendrocyte specific bHLH transcription factor 2 Oligodendrocyte transcription factor 2 OTTHUMP00000067569 OTTHUMP00000067570 PRKCBP2 Protein kinase C binding protein 2 Protein kinase C binding protein RACK17 Protein kinase C-binding protein 2 Protein kinase C-binding protein RACK17 RACK17 |
|
Fig1:
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Olig2 antibody (HA601558) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601558) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-Olig2 antibody (HA601558) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601558) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |