| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P |
| Clone number: | PSH16-52 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 11 kDa |
| Positive control: | Mouse brain tissue, rat brain tissue. |
| Subcellular location: | Secreted, Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle. |
| Recommended Dilutions:
IHC-Fr IHC-P |
1:200 1:500 |
| Uniprot #: | SwissProt: P57774 Mouse | P07808 Rat |
| Alternative names: | C-flanking peptide of NPY CPON Neuropeptide tyrosine Neuropeptide Y precursor NPY NPY_HUMAN Pro neuropeptide Y PYY 4 PYY4 Y Neuropeptide |
|
Fig1:
Application: IHC-Fr Species: Mouse Site: cerebral cortex Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Chicken anti-Neuropeptide Y antibody (HA601559) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601559) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Chicken anti-Neuropeptide Y antibody (HA601559) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601559) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |