Calbindin Recombinant Antibody [JF05-01] - Guinea pig IgG2 (Chimeric)
cat.: HA601562
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, WB |
| Clone number: | JF05-01 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Immunogen: | Synthetic peptide within Human Calbindin aa 51-100 / 261. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate. |
| Subcellular location: | Cytosol, Nucleus. |
| Recommended Dilutions:
IHC-Fr WB |
1:500 1:5,000 |
| Uniprot #: | SwissProt: P12658 Mouse | P07171 Rat |
| Alternative names: | avian-type CAB27 CALB 1 CALB CALB1 CALB1_HUMAN Calbindin 1 28kDa Calbindin Calbindin D28 D 28K D-28K D28K OTTHUMP00000166027 OTTHUMP00000225441 RTVL H protein Vitamin D dependent calcium binding protein Vitamin D dependent calcium binding protein avian type Vitamin D-dependent calcium-binding protein |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Western blot analysis of Calbindin on different lysates with Guinea pig anti-Calbindin antibody (HA601562) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Rat brain tissue lysate (20 µg/Lane) Lane 3: C6 cell lysate (negative) (10 µg/Lane) Lane 4: Mouse kidney tissue lysate (20 µg/Lane) Lane 5: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 30 kDa Observed band size: 30 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601562) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.