Vimentin Recombinant Antibody - Rat IgG1 (Chimeric)
cat.: HA601564
| Product Type: | Recombinant Chimeric Antibody IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, IF-Cell, mIHC |
| Clone number: | PDH0-01 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.5ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within C-terminal human Vimentin. |
| Positive control: | Human Appendix tissue, HeLa. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
IHC-P IF-Cell mIHC |
1:1,000 1:100 1:2,500-1:4,000 |
| Uniprot #: | SwissProt: P08670 Human |
| Alternative names: | CTRCT30 Epididymis luminal protein 113 FLJ36605 HEL113 VIM VIME_HUMAN Vimentin |
Images
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Fig1:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Appendix Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601564, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1023, 30 minutes at room temperature. |
|
Fig2:
Application: Immunocytochemistry (IF-cell) Species: Human Sample: HeLa (Human cervix adenocarcinoma epithelial cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA601564, 1/100, overnight at 4℃. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature. Counterstain: Beta tubulin (ET1602-4, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.