Beta Catenin Recombinant Antibody - Rat IgG1 (Chimeric)
cat.: HA601570
Product Type: Recombinant Chimeric Antibody, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-Fr, IHC-P, IF-Cell, mIHC
Clone number: SA30-04
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 85 kDa
Immunogen: Synthetic peptide within human Beta-Catenin aa 30-70.
Positive control: HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human colon cancer tissue, human colon tissue, mouse colon tissue, rat colon tissue, C6.
Subcellular location: Cytoplasm, Nucleus, Cell membrane, Cell junction
Recommended Dilutions:
  WB
  IHC-Fr
  IHC-P
  IF-Cell
  mIHC
1:2,000-1:5,000
1:200
1:200-1:1,000
1:100
1:1,000
Uniprot #: SwissProt: P35222 Human | Q02248 Mouse | Q9WU82 Rat
Alternative names: β Catenin Beta catenin Beta-catenin Cadherin associated protein Catenin (cadherin associated protein), beta 1, 88kDa Catenin beta 1 Catenin beta-1 CATNB CHBCAT CTNB1_HUMAN CTNNB CTNNB1 DKFZp686D02253 FLJ25606 FLJ37923 OTTHUMP00000162082 OTTHUMP00000165222 OTTHUMP00000165223 OTTHUMP00000209288 OTTHUMP00000209289
Images
HA601570_1.jpg Fig1: Western blot analysis of Beta Catenin on different lysates with Rat anti-Beta Catenin antibody (HA601570) at 1/5,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: C6 cell lysate (20 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 5: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 85 kDa
Observed band size: 85 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601570) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.
HA601570_2.jpg Fig2: Application: IHC-Fr

Species: Mouse

Site: colon

Sample: Frozen section

Antibody concentration: 1/200

Antigen retrieval: Not required
HA601570_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rat anti-Beta Catenin antibody (HA601570) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601570) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601570_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rat anti-Beta Catenin antibody (HA601570) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601570) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601570_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rat anti-Beta Catenin antibody (HA601570) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601570) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601570_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rat anti-Beta Catenin antibody (HA601570) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601570) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601570_7.jpg Fig7: Immunocytochemistry analysis of C6 cells labeling Beta Catenin with Rat anti-Beta Catenin antibody (HA601570) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rat anti-Beta Catenin antibody (HA601570) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.