| Product Type: | Recombinant Rat Monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | FC |
| Clone number: | PSH20-65 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Positive control: | BALB/c mouse splenocytes. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
FC |
1:2,000 |
| Uniprot #: | SwissProt: P08508 Mouse | P08101 Mouse |
| Alternative names: | CD16a CD16b CD32 CD32A CD32B CDw32 Fc fragment of IgG low affinity IIa receptor Fc fragment of IgG low affinity IIb receptor Fc fragment of IgG low affinity IIIa receptor Fc fragment of IgG low affinity IIIb receptor Fc gamma receptor III A Fc gamma receptor IIIb Fc gamma RIIa Fc gamma RIIb Fc gamma RIII beta Fc-gamma RII-a Fc-gamma-RIIa FCG2A_HUMAN FcGR FCGR2A FCGR2B FCGR3 FCGR3A FCGR3B FcRII-a FcRIII IgG Fc receptor II-a Low affinity immunoglobulin gamma Fc region receptor II-a |
|
Fig1:
Flow cytometric analysis of BALB/c mouse splenocytes labeling CD16/CD32. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601571, 1/2,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 594 conjugate-Goat anti-Rat IgG Secondary antibody (HA1136) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |