Myelin Basic Protein Recombinant Antibody [JF0943] - Chicken IgY (Chimeric)
cat.: HA601580
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-Fr, IF-Tissue |
| Clone number: | JF0943 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Immunogen: | Recombinant protein within Human Myelin Basic Protein aa 121-304 / 304. |
| Positive control: | Human brain tissue, mouse brain tissue, rat brain tissue, |
| Subcellular location: | Myelin membrane, Nucleus. |
| Recommended Dilutions:
IHC-Fr IF-Tissue |
1:200 1:500 |
| Uniprot #: | SwissProt: P02686 Human | P04370 Mouse | P02688 Rat |
| Alternative names: | GDB Golli MBP Golli MBP; myelin basic protein Hemopoietic MBP HMBPR HUGO MBP MBP_CAVPO MBP_HUMAN MGC99675 MLD Myelin A1 protein Myelin A1 Protein, basic Myelin basic protein Myelin Deficient Myelin membrane encephalitogenic protein OTTHUMP00000163776 OTTHUMP00000174387 OTTHUMP00000174388 SHI Shiverer SP |
Images
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Fig1:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Cerebral cortex Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× PBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA601580, 1/200, overnight at 4℃. Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature. |
|
Fig2:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Hippocampus Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× PBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA601580, 1/200, overnight at 4℃. Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature. |
|
Fig3:
Application: Immunofluorescence (IF-tissue) Species: Human Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601580, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature. |
|
Fig4:
Application: Immunofluorescence (IF-tissue) Species: Mouse Tissue: Cerebral cortex Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601580, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature. |
|
Fig5:
Application: Immunofluorescence (IF-tissue) Species: Rat Tissue: Cerebral cortex Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601580, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.