Myogenin Recombinant Mouse Monoclonal Antibody [PSH22-35]
cat.: HA601587
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | PSH22-35 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG1 |
| Positive control: | RD cell lysate, C2C12 cell lysate, C2C12 treated with 2% horse serum for 24 hours cell lysate, RD, rat embryo tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P IHC-Fr |
1:2,000 1:100 1:500 1:200 |
| Uniprot #: | SwissProt: P15173 Human | P12979 Mouse | P20428 Rat |
| Alternative names: | bHLHc3 cb553 Class C basic helix-loop-helix protein 3 MYF 4 Myf-4 MYF4 MYOG MYOG_HUMAN Myogenic factor 4 Myogenic factor 4 myogenin Myogenin Myogenin myogenin factor 4 OTTHUMP00000039094 |
Images
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Fig1:
Western blot analysis of Myogenin on different lysates with Mouse anti-Myogenin antibody (HA601587) at 1/2,000 dilution. Lane 1: RD cell lysate Lane 2: HeLa cell lysate (negative) Lane 3: C2C12 cell lysate Lane 4: C2C12 treated with 2% horse serum for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 34 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601587) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of RD (positive) and HeLa (negative) labeling Myogenin with Mouse anti-Myogenin antibody (HA601587) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Myogenin antibody (HA601587) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Mouse anti-Myogenin antibody (HA601587) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601587) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Application: IHC-Fr Species: Rat Site: embryo Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.