Myelin Basic Protein Recombinant Antibody [JF0943] - Guinea pig IgG2 (Chimeric)
cat.: HA601600
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, WB |
| Clone number: | JF0943 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Immunogen: | Recombinant protein within Human Myelin Basic Protein aa 121-304 / 304. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Myelin membrane, Nucleus. |
| Recommended Dilutions:
IHC-Fr WB |
1:500 1:20,000 |
| Uniprot #: | SwissProt: P04370 Mouse | P02688 Rat |
| Alternative names: | GDB Golli MBP Golli MBP; myelin basic protein Hemopoietic MBP HMBPR HUGO MBP MBP_CAVPO MBP_HUMAN MGC99675 MLD Myelin A1 protein Myelin A1 Protein, basic Myelin basic protein Myelin Deficient Myelin membrane encephalitogenic protein OTTHUMP00000163776 OTTHUMP00000174387 OTTHUMP00000174388 SHI Shiverer SP |
Images
|
Fig1:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: Cerebral cortex Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× PBS Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA601600, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature. |
|
Fig2:
Western blot analysis of Myelin Basic Protein on different lysates with Guinea pig anti-Myelin Basic Protein antibody (HA601600) at 1/20,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601600, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Rabbit anti-Guinea pig IgG-HRP (HA1021), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 33 kDa Observed band size: 14-20 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.