HRP Conjugated Monkey PCSK9 Recombinant Mouse Monoclonal Antibody [PSH23-55]
cat.: HA601603H
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Cynomolgus monkey |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH23-55 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Cynomolgus PCSK9 aa 31-692 (HA211416). |
| Positive control: | Recombinant Cynomolgus PCSK9 protein (HA211416). |
| Subcellular location: | Cytoplasm, Secreted, Endosome, Lysosome, Cell surface, Endoplasmic reticulum, Golgi apparatus. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-54] to Monkey PCSK9 (Capture) (HA724385) and Recombinant Cynomolgus PCSK9 protein (HA211416) as the standard. The reference range value is 390.6-50,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: G7NVZ1 Monkey |
| Alternative names: | Convertase subtilisin/kexin type 9 preproprotein FH3 HCHOLA3 Hypercholesterolemia autosomal dominant 3 LDLCQ1 NARC 1 NARC-1 NARC1 Neural apoptosis regulated convertase 1 Neural apoptosis-regulated convertase 1 PC 9 PC9 PCSK 9 PCSK9 Proprotein convertase 9 Proprotein convertase PC9 Proprotein convertase subtilisin/kexin type 9 PSEC0052 Subtilisin/kexin like protease PC9 Subtilisin/kexin-like protease PC9 |
Images
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Fig1:
Sandwich ELISA analysis of Cynomolgus PCSK9 matched pair antibodies Capture: HA724385, Monkey PCSK9 Rabbit mAb [PSH23-54] Detector: HA601603, Monkey PCSK9 Mouse mAb [PSH23-55] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724385) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Cynomolgus PCSK9 protein (HA211416) starting from 50,000 pg/ml to 0 pg/ml and detect antibody (HA601603, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.