CD21 Recombinant Antibody [PD00-23] - Rat IgG1 (Chimeric)
cat.: HA601605
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clone number: | PD00-23 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 113 kDa |
| Immunogen: | Synthetic peptide within Human CD21 aa 1000 to the C-terminus. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:4,000 |
| Uniprot #: | SwissProt: P20023 Human |
| Alternative names: | C3DR CD 21 CD21 Complement C3d receptor 2 Complement C3d receptor Complement component (3d/Epstein Barr virus) receptor 2 Complement receptor type 2 CR Cr2 CR2_HUMAN CVID7 EBV receptor EBV-R Epstein Barr virus receptor Epstein-Barr virus receptor EVBR SLEB9 |
Images
|
Fig1:
Western blot analysis of CD21 on different lysates with Rabbit anti-CD21 antibody (HA601605) at 1/5,000 dilution. Lane 1: Raji (Human Burkitt's lymphoma cells) Lane 2: K-562 (Human chronic myelogenous leukemia cells) Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Negative expression of CD21 protein in K562 cell is consistent with the predicted expression pattern Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601605, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,00 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 113 kDa Observed band size: 150 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Lymph node Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601605, 1/4,000, 1 hour at room temperature. Secondary antibody: HA1023, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601605, 1/4,000, 1 hour at room temperature. Secondary antibody: HA1023, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.