GAD67 Recombinant Antibody [PSH08-30] - Guinea pig IgG2 (Chimeric)
cat.: HA601606
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clone number: | PSH08-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa |
| Immunogen: | Recombinant protein within human GAD67 aa 1-594. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, Plasma Membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:5,000 1:500 1:500 |
| Uniprot #: | SwissProt: Q99259 Human | P48318 Mouse | P18088 Rat |
| Alternative names: | 67 kDa glutamic acid decarboxylase CPSQ1 DCE1 DCE1_HUMAN EC 4.1.1.15 FLJ45882 GAD 67 GAD GAD-67 GAD1 Glutamate decarboxylase 1 (brain, 67kDa) Glutamate decarboxylase 1 Glutamate decarboxylase 1 brain 67kD Glutamate decarboxylase 1 brain 67kDa Glutamate decarboxylase 67 kDa isoform Glutamate decarboxylase, brain, 67-KD OTTHUMP00000041055 SCP |
Images
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Fig1:
Western blot analysis of GAD67 on different lysates with Guinea pig anti-GAD67 antibody (HA601606) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate (negative) Lane 3: Rat brain tissue lysate Lane 4: Rat liver tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Liver is a negative control (PMID: 33968199). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601606, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Guinea pig IgG-HRP (HA1021), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 67 kDa Observed band size: 67 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Cerebellum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601606, 1/500, 1 hour at room temperature. Secondary antibody: HA1021, 30 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Hippocampus Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601606, 1/500, 1 hour at room temperature. Secondary antibody: HA1021, 30 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Cerebral cortex Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601606, 1/500, 1 hour at room temperature. Secondary antibody: HA1021, 30 minutes at room temperature. |
|
Fig5:
Application: Immunofluorescence (IF-tissue)
Species: Mouse Tissue: Hippocampus Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× PBS Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Antibody A: 1/500 (GAD67, HA601606, guinea pig, Red), Antibody B: 1/500 (VGLUT1, HA723217, rabbit, Green), overnight at 4°C. Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), Goat anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig6:
Application: Immunofluorescence (IF-tissue)
Species: Rat Tissue: Hippocampus Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× PBS Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Antibody A: 1/500 (GAD67, HA601606, guinea pig, Red), Antibody B: 1/500 (VGLUT1, HA723217, rabbit, Green), overnight at 4°C. Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), Goat anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.