SQSTM1 / p62 Recombinant Antibody [PS00-61] - Rat IgG1 (Chimeric)
cat.: HA601610
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clone number: | PS00-61 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Immunogen: | Synthetic peptide within Human SQSTM1/ p62 aa 400 to the C-terminus |
| Subcellular location: | Cytoplasm, Endoplasmic reticulum, Endosome, Lysosome, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:50,000 1:500-1:2,000 |
| Uniprot #: | SwissProt: Q13501 Human | Q64337 Mouse | O08623 Rat |
| Alternative names: | A170 DMRV EBI 3 associated protein of 60 kDa EBI 3 associated protein p60 EBI3 associated protein of 60 kDa EBI3 associated protein p60 EBI3-associated protein of 60 kDa EBIAP FTDALS3 MGC127197 ORCA OSF-6 Osi OSIL Oxidative stress induced like p60 p62 p62B Paget disease of bone 3 PDB 3 PDB3 Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa Phosphotyrosine independent ligand for the Lck SH2 domain p62 Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa PKC-zeta-interacting protein Protein kinase C-zeta-interacting protein Sequestosome 1 Sequestosome-1 SQSTM 1 SQSTM_HUMAN Sqstm1 STAP STONE14 Ubiquitin binding protein p62 Ubiquitin-binding protein p62 ZIP 3 ZIP ZIP3 |
Images
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Fig1:
Western blot analysis of SQSTM1 / p62 on different lysates with Rat anti-SQSTM1 / p62 antibody (HA601610) at 1/50,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 2: PANC-1 (Human epidermoid carcinoma pancreatic ductal cells) cell lysate Lane 3: C2C12 (Mouse myoblasts) cell lysate Lane 4: RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate Lane 5: PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601610, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 48 kDa Observed band size: 62 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601610, 1/500, 1 hour at room temperature. Secondary antibody: HA1023, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601610, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1023, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601610, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1023, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.