PARK7 Recombinant Mouse Monoclonal Antibody [PSH23-77]
cat.: HA601612
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH23-77 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG1 |
| Positive control: | HeLa (Human cervical adenocarcinoma cells) cell lysate, A549 (Human lung adenocarcinoma cells) cell lysate, SH-SY5Y (Human neuroblastoma cells) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, C6 (Rat glioma cells) cell lysate, Mouse brain (4weeks) tissue lysate, Rat brain (4weeks) tissue lysate. |
| Subcellular location: | Cell membrane, Cytoplasm, Nucleus, Membrane raft, Mitochondrion, Endoplasmic reticulum. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:1,000-1:5,000 |
| Uniprot #: | SwissProt: Q99497 Human | Q99LX0 Mouse | O88767 Rat |
| Alternative names: | CAP1 DJ-1 DJ1 DJ1 protein Epididymis secretory sperm binding protein Li 67p FLJ27376 FLJ34360 FLJ92274 HEL S 67p Oncogene DJ1 OTTHUMP00000001348 OTTHUMP00000001349 OTTHUMP00000001350 OTTHUMP00000001351 PARK7 PARK7_HUMAN Parkinson disease (autosomal recessive, early onset) 7 Parkinson disease protein 7 Parkinson protein 7 Protein DJ-1 SP22 |
Images
|
Fig1:
Western blot analysis of PARK7 on different lysates with Mouse anti-PARK7 antibody (HA601612) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 2: A549 (Human lung adenocarcinoma cells) cell lysate Lane 3: SH-SY5Y (Human neuroblastoma cells) cell lysate Lane 4: NIH/3T3 (Mouse fibroblasts) cell lysate Lane 5: C6 (Rat glioma cells) cell lysate Lane 6: Mouse brain (4weeks) tissue lysate Lane 7: Rat brain (4weeks) tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601612, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 20 kDa Observed band size: 20 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601612, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601612, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601612, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.