PAX5 Recombinant Antibody [PSH06-99] - Mouse IgG1 (Chimeric)
cat.: HA601616
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P |
| Clone number: | PSH06-99 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Immunogen: | Recombinant protein within human PAX5 aa 101-391. |
| Positive control: | Human tonsil tissue, mouse spleen tissue, rat spleen tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-P |
1:2,000 |
| Uniprot #: | SwissProt: Q02548 Human | Q02650 Mouse Entrez Gene: 500453 Rat |
| Alternative names: | B cell lineage specific activator B cell lineage specific activator protein B cell specific activator protein B cell specific transcription factor B-cell-specific transcription factor BSAP EBB-1 KLP Paired box 5 Paired box gene 5 (B cell lineage specific activator protein) Paired box gene 5 (B cell lineage specific activator) Paired box gene 5 Paired box homeotic gene 5 Paired box protein Pax 5 Paired box protein Pax-5 Paired domain gene 5 PAX 5 PAX5 PAX5_HUMAN Transcription factor PAX 5 |
Images
|
Fig1:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Tonsil Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, sodium citrate buffer (pH 6.0), 2 minutes at high pressure. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601616, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, sodium citrate buffer (pH 6.0), 2 minutes at high pressure. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601616, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, sodium citrate buffer (pH 6.0), 2 minutes at high pressure. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601616, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.