Bassoon / BSN Recombinant Mouse Monoclonal Antibody [PSH24-11]
cat.: HA601618
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH24-11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 419 kDa |
| Isotype: | IgG1 |
| Positive control: | Mouse cerebellum tissue, rat cerebellum tissue. |
| Subcellular location: | Cytoplasm, Presynaptic active zone, cytoskeleton, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane |
| Recommended Dilutions:
IHC-Fr IHC-P |
1:500 1:1,000 |
| Uniprot #: | SwissProt: O88737 Mouse | O88778 Rat |
| Alternative names: | Protein bassoon Bsn Kiaa0434 |
Images
|
Fig1:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Cerebellum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601618, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Heart Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601618, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. Negative control: Heart (PMID: 9679147) |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Cerebellum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601618, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Heart Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601618, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. Negative control: Heart (PMID: 9679147) |
|
Fig5:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: Cerebellum Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA601618, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.