TDP43 Recombinant Antibody [PSH09-14] - Rat IgG1 (Chimeric)
cat.: HA601627
Product Type: Recombinant Chimeric Antibody, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Tissue, IF-Cell
Clone number: PSH09-14
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 45 kDa
Immunogen: Synthetic peptide within human TDP43 aa 365-414.
Positive control: HeLa (Human cervical adenocarcinoma cell) lysate, HEK-293 (Human embryonic kidney cell) lysate, SH-SY5Y (Human neuroblastoma cell) lysate, K-562 (Human chronic myelogenous leukemia cell) lysate, C2C12 (Mouse myoblast) lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) lysate, Mouse brain tissue lysate.
Subcellular location: Cytoplasm, Mitochondrion, Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Tissue
  IF-Cell
1:2,000
1:500-1:2,000
1:500
1:50
Uniprot #: SwissProt: Q13148 Human | Q921F2 Mouse
Entrez Gene: 298648 Rat
Alternative names: ALS10 OTTHUMP00000002171 OTTHUMP00000002172 OTTHUMP00000002173 TADBP_HUMAN TAR DNA binding protein 43 TAR DNA binding protein TAR DNA-binding protein 43 TARDBP TDP 43 TDP-43 TDP43
Images
HA601627_1.jpg Fig1: Western blot analysis of TDP43 on different lysates with Rat anti-TDP43 antibody (HA601627) at 1/2,000 dilution.

Lane 1: HeLa (Human cervical adenocarcinoma cell) lysate
Lane 2: HEK-293 (Human embryonic kidney cell) lysate
Lane 3: SH-SY5Y (Human neuroblastoma cell) lysate
Lane 4: K-562 (Human chronic myelogenous leukemia cell) lysate
Lane 5: C2C12 (Mouse myoblast) lysate
Lane 6: PC-12 (Rat pheochromocytoma cell (undifferentiated)) lysate
Lane 7: Mouse brain tissue lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 20 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601627, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 45 kDa
Observed band size: 45 kDa
HA601627_2.jpg Fig2: Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Brain
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601627, 1/500, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature.
HA601627_3.jpg Fig3: Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601627, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature.
HA601627_4.jpg Fig4: Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601627, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature.
HA601627_5.jpg Fig5: Application: Immunofluorescence (IF-tissue)

Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601627, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature.
HA601627_6.jpg Fig6: Application: Immunofluorescence (IF-tissue)

Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601627, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature.
HA601627_7.jpg Fig7: Application: Immunocytochemistry (IF-cell)

Species: Human
Sample: HeLa (Human cervix adenocarcinoma epithelial cell)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.

Primary antibody: HA601627, 1/50, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.
Counterstain: Beta tubulin (ET1602-4, Red), 1/200, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
HA601627_8.jpg Fig8: Application: Immunocytochemistry (IF-cell)

Species: Mouse
Sample: C2C12 (Mouse myoblast)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.

Primary antibody: HA601627, 1/50, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.
Counterstain: Beta tubulin (ET1602-4, Red), 1/200, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.