Ganglioside GD2 Recombinant Mouse Monoclonal Antibody [PSH24-41]
cat.: HA601628
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, FC(Intra) |
| Clonality: | Monoclonal |
| Clone number: | PSH24-41 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Positive control: | IMR-32. |
| Recommended Dilutions:
IF-Cell FC(Intra) |
1:500 1:1,000 |
Images
|
Fig1:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: IMR-32 (Human neuroblastoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA601628, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature. Counterstain: Beta tubulin (ET1602-4, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
|
Fig2:
Application: Flow Cytometry (Intra)
Species: Human Sample: IMR-32 (Human neuroblastoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA601628(1/1,000, Red) compared with Mouse IgG1 Isotype Control (HA601336, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.