Cytokeratin 19 Recombinant Antibody [SA30-06] - Mouse IgG1 (Chimeric)
cat.: HA601630
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IHC-P, IF-Cell |
| Clone number: | SA30-06 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Immunogen: | Synthetic peptide within Human Cytokeratin 19 aa 348-400 / 400. |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
IHC-P IF-Cell |
1:1,000 1:500 |
| Uniprot #: | SwissProt: P08727 Human | P19001 Mouse |
| Alternative names: | 40 kDa keratin intermediate filament CK 19 CK-19 CK19 Cytokeratin 19 Cytokeratin-19 K19 K1C19_HUMAN K1CS Keratin 19 Keratin type I 40 kD Keratin type I 40kD Keratin type I cytoskeletal 19 Keratin, type I cytoskeletal 19 Keratin, type I, 40 kd Keratin-19 KRT19 MGC15366 |
Images
|
Fig1:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601630, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601630, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601630, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601630, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig5:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: MCF7 (Human breast cancer cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA601630, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature. Counterstain: beta Tubulin (ET1602-4, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.