CD16 Recombinant Antibody [PD00-12] - Mouse IgG1 (Chimeric)
cat.: HA601634
Product Type: Recombinant Chimeric Antibody, primary antibodies
Species reactivity: Human
Applications: IHC-P, IF-Tissue
Clone number: PD00-12
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 29 kDa
Immunogen: Recombinant protein within human CD16 aa 17-208 (Extracellular).
Subcellular location: Cell membrane, Secreted.
Recommended Dilutions:
  IHC-P
  IF-Tissue

1:1,000
1:500
Uniprot #: SwissProt: P08637 Human
Alternative names: CD 16 CD 16a CD16 CD16a CD16a antigen CD16B CD16b antigen Fc fragment of IgG Fc fragment of IgG low affinity IIIa receptor (CD16) Fc fragment of IgG low affinity IIIa receptor Fc fragment of IgG receptor IIIa Fc fragment of IgG, low affinity III, receptor (CD16) Fc fragment of IgG, low affinity III, receptor for (CD16) Fc fragment of IgG, low affinity IIIa, receptor (CD16) Fc fragment of IgG, low affinity IIIa, receptor (CD16a) Fc fragment of IgG, low affinity IIIa, receptor for Fc fragment of IgG, low affinity IIIb, receptor (CD16b) Fc fragment of IgG, low affinity IIIb, receptor for (CD16) Fc gamma R3 Fc gamma receptor III 2 (CD 16) Fc gamma receptor III A Fc gamma receptor IIIA Fc gamma receptor IIIb (CD 16) Fc gamma RIII alpha Fc gamma RIII Fc gamma RIII beta Fc gamma RIIIa Fc gamma RIIIb Fc of IgG Fc-gamma receptor III2 (CD 16) Fc-gamma receptor III2 (CD16) Fc-gamma receptor IIIb (CD16) Fc-gamma RIII Fc-gamma RIII-a......
Images
HA601634_1.jpg Fig1: Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Spleen
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601634, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature.
HA601634_2.jpg Fig2: Application: Immunofluorescence (IF-tissue)

Species: Human
Tissue: Spleen
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601634, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.