iFluor™ 647 Conjugated Vimentin Mouse Monoclonal Antibody [D4-B11]
cat.: HA601646F
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | D4-B11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within C-terminal human Vimentin. |
| Positive control: | Human skin tissue, human kidney tissue, HeLa. |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
IF-Tissue FC |
1:100 1 μg/mL |
| Uniprot #: | SwissProt: P08670 Human |
| Alternative names: | CTRCT30 Epididymis luminal protein 113 FLJ36605 HEL113 VIM VIME_HUMAN Vimentin |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Vimentin with Mouse anti-Vimentin antibody (HA601646F) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601646F, red) at 1/100 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue). |
|
Fig2:
Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Vimentin with Mouse anti-Vimentin antibody (HA601646F) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601646F, red) at 1/100 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue). |
|
Fig3:
Flow cytometric analysis of HeLa cells labeling Vimentin. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Vimentin (HA601646F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.