Progesterone Receptor Recombinant Antibody [JF0549] - Mouse IgG1 (Chimeric)
cat.: HA601647
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, IHC-P |
| Clone number: | JF0549 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 99 kDa |
| Immunogen: | Synthetic peptide within N-terminal human Progesterone Receptor. |
| Positive control: | T-47D (Human breast ductal carcinoma cell, Human Breast cancer. |
| Subcellular location: | Nucleus, Cytoplasm, Mitochondrion outer membrane. |
| Recommended Dilutions:
IF-Cell IHC-P |
1:500 1:1,000 |
| Uniprot #: | SwissProt: P06401 Human |
| Alternative names: | NR3C3 Nuclear receptor subfamily 3 group C member 3 PGR PR PRA PRB PRGR_HUMAN Progesterone receptor Progestin receptor form A Progestin receptor form B |
Images
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Fig1:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: T-47D (Human breast ductal carcinoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA601647, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature. Counterstain: Beta tubulin (ET1602-4, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Breast cancer Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601647, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.