Rabbit IgG heavy chain(Fc fragment) Recombinant Mouse Monoclonal Antibody [PSH23-92]
cat.: HA601651
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Rabbit |
| Applications: | WB, ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH23-92 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 5ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 50 kDa |
| Isotype: | IgG1 |
| Immunogen: | Purified Rabbit IgG heavy chain(Fc fragment). |
| Subcellular location: | secrete |
| Recommended Dilutions:
WB ELISA |
1:5,000 1:5,000 |
Images
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Fig1:
Western blot analysis of Rabbit IgG heavy chain(Fc fragment) on Rabbit IgG (positive) with Mouse anti-Rabbit IgG heavy chain(Fc fragment) antibody (HA601651) at 1/50,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601651, 1/50,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Observed band size: 50 kDa |
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Fig2:
Western blot analysis of Rabbit IgG heavy chain(Fc fragment) on Mouse IgG (negative) with Mouse anti-Rabbit IgG heavy chain(Fc fragment) antibody (HA601651) at 1/50,000 dilution.
Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601651, 1/50,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature |
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Fig3:
Western blot analysis of Rabbit IgG heavy chain(Fc fragment) on Human IgG (negative) with Mouse anti-Rabbit IgG heavy chain(Fc fragment) antibody (HA601651) at 1/50,000 dilution.
Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601651, 1/50,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature |
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Fig4: Indirect ELISA analysis of Rabbit IgG heavy chain(Fc fragment) was performed by coating wells of a 96-well plate with 50 µL per well of Fc fragment of Rabbit lgG / F(ab')₂ fragment of Rabbit IgG / Human IgG diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer for 1 hour at 37℃, and incubated with 50 µL per well of Rabbit IgG heavy chain(Fc fragment) recombinant Mouse monoclonal antibody serial diluted starting from a concentration of 4µg/mL for 45 minutes at 37℃. The plate was washed and incubated with 50 µL per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1/80,000 for 30 minutes at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.