HRP Conjugated DYKDDDDK Tag (FLAG) Recombinant Mouse Monoclonal Antibody [A2-A4-R]
cat.: HA601652H
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A2-A4-R |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.05ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide immune sequence is N-DYKDDDDK-C. |
| Recommended Dilutions:
WB |
1:2,000 |
| Alternative names: | DDDDK epitope tag DDDK ddk DYKDDDDK DYKDDDDK epitope tag DYKDDDDK tag ECS epitope tag ECS tag Enterokinase Cleavage Site epitope tag Enterokinase Cleavage Site tag FLAG FLAG tag |
Images
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Fig1:
Western blot analysis of DYKDDDDK Tag (FLAG) on different lysates with Mouse anti-DYKDDDDK Tag (FLAG) antibody (HA601652H) at 1/2,000 dilution.
Lane 1: 293T (Human embryonic kidney cell) transfected with DYKDDDDK-tagged Histone H3.1 (N-terminal) cell lysate Lane 2: 293T transfected with DYKDDDDK-tagged Depdc7 (C-terminal) cell lysate Lane 3: 293T transfected with DYKDDDDK-tagged Rela (C-terminal) cell lysate Lysates/proteins at 5 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601652H, 1/2,000 in primary antibody dilution buffer (K1803), 1 hour at room temperature |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.