Beta III Tubulin Recombinant Antibody [PSH15-32] - Mouse IgG1 (Chimeric)
cat.: HA601654
Product Type: Recombinant Chimeric Antibody, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IHC-P, IF-Tissue, IHC-Fr, WB, IF-Cell
Clone number: PSH15-32
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 0.5ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 50 kDa
Immunogen: Synthetic peptide within mouse Beta III Tubulin aa 401-450.
Positive control: Neuro-2a, Human Cerebellum, Mouse Cerebellum, Rat Cerebellum.
Subcellular location: Cytoplasm, cytoskeleton, growth cone, lamellipodium, filopodium.
Recommended Dilutions:
  WB
  IHC-P
  IF-Tissue
  IHC-Fr
  IF-Cell
1:10,000
1:2,000
1:1,000
1:200-1:500
1:50
Uniprot #: SwissProt: Q13509 Human | Q9ERD7 Mouse | Q4QRB4 Rat
Alternative names: beta 3 tubulin beta 4 beta-4 CDCBM CDCBM1 CFEOM3 CFEOM3A FEOM3 M(beta)3 M(beta)6 MC1R Neuron specific beta III Tubulin Neuron-specific class III beta-tubulin QccE-11995 QccE-15186 TBB3_HUMAN Tubb 3 TUBB3 TUBB4 Tubulin beta 3 Tubulin beta 3 chain Tubulin beta 4 Tubulin beta III Tubulin beta-3 chain Tubulin beta-4 chain Tubulin beta-III tuj 1 tuj1
Images
HA601654_1.jpg Fig1: Application: Immunofluorescence (IHC-Fr)

Species: Mouse
Tissue: Cerebral cortex
Sample: Frozen section

Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.

Primary antibody: HA601654, 1/250, overnight at 4℃.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature.
HA601654_2.jpg Fig2: Application: Immunofluorescence (IHC-Fr)

Species: Rat
Tissue: Cerebral cortex
Sample: Frozen section

Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.

Primary antibody: HA601654, 1/250, overnight at 4℃.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature.
HA601654_3.jpg Fig3: Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Cerebellum
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601654, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature.
HA601654_4.jpg Fig4: Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Cerebellum
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601654, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature.
HA601654_5.jpg Fig5: Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Cerebellum
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601654, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature.
HA601654_6.jpg Fig6: Application: Immunofluorescence (IF-tissue)

Species: Human
Tissue: Cerebellum
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601654, 1/1,000, overnight at 4°C.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature.
HA601654_7.jpg Fig7: Application: Immunofluorescence (IF-tissue)

Species: Mouse
Tissue: Cerebellum
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601654, 1/1,000, overnight at 4°C.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature.
HA601654_8.jpg Fig8: Application: Immunofluorescence (IF-tissue)

Species: Rat
Tissue: Cerebellum
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601654, 1/1,000, overnight at 4°C.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature.
HA601654_9.jpg Fig9: Application: Immunocytochemistry (IF-cell)

Species: Human
Sample: Neuro-2a (Mouse brain neuroblastoma cell)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.

Primary antibody: HA601654, 1/50, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (ET1602-4, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
HA601654_10.JPG Fig10: Western blot analysis of Beta III Tubulin on different lysates with Mouse anti-Beta III Tubulin antibody (HA601654) at 1/10,000 dilution.

Lane 1: SH-SY5Y (Human neuroblastoma cell)
Lane 2: HeLa (Human cervical adenocarcinoma cell)(Low expression)
Lane 3: Neuro-2a (Mouse brain neuroblastoma cell)
Lane 4: NIH/3T3 (Mouse fibroblast)(negative)

Lysates/proteins at 10 µg/Lane.
Exposure time: 20 seconds; ECL: K1801

HeLa is a relative low expression control (PMID: 35573698)
and NIH/3T3 is a negative control (PMID: 25839941).

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601654, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 50 kDa
Observed band size: 50 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.